Fluorescence spectroscopy is another PKU screening method which measures the phenylalanine (Phe) level in the blood. This type of electromagnetic spectroscopy analyzes fluoroscence from a blood sample, which is exposed to a beam of (usually) ultraviolet light.
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Tandem mass spectrometry is using tandem mass spectrometers – instruments that have more than one analyser and can quickly sort biochemically molecules of similiar weight. PKU screening test with Tandem mass spectrometry method is more reliable than other tests and reduces the number of falsely positive PKU results.
Instead of red-brown urine color reaction and ketones discovery, the girls’ urine sample assumed a dark-green color which faded in a few minutes. Repeated tests after two weeks gave Dr. Følling the same results, which further intrigued him to finally find and isolate the main source of this unusual chemical reaction.
Did you know that all the newborns since 1970s’ have had at least one Phenylketonuria (PKU) test done in their life? And almost none of them knew that they’ve been tested on this disease. Namely, from 1966., most of the countries started to organize neonatal screenings. That means that every child was taken a little bit of blood from the heel after the birth, and then was tested on PKU disease.